Mixing
How can I set micropipette volume with decimal separators, for example 109.5 in p200?
$begingroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
edited Jan 15 at 9:24
Laurel
1854
asked Jan 14 at 18:09
SaraSara
141
$endgroup$
add a comment |
$begingroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
edited Jan 15 at 9:24
Laurel
1854
asked Jan 14 at 18:09
SaraSara
141
$endgroup$
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51
add a comment |
$begingroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
edited Jan 15 at 9:24
Laurel
1854
asked Jan 14 at 18:09
SaraSara
141
$endgroup$
And how can I set:
- 150.1 in p200
- 871.3 in p1000
- 840.5 in p1000?
molecular-biology
molecular-biology
edited Jan 15 at 9:24
Laurel
1854
asked Jan 14 at 18:09
SaraSara
141
edited Jan 15 at 9:24
Laurel
1854
asked Jan 14 at 18:09
SaraSara
141
edited Jan 15 at 9:24
Laurel
1854
edited Jan 15 at 9:24
Laurel
1854
edited Jan 15 at 9:24
Laurel
1854
1854
asked Jan 14 at 18:09
SaraSara
141
asked Jan 14 at 18:09
SaraSara
141
asked Jan 14 at 18:09
SaraSara
141
141
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51
add a comment |
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51
$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51
add a comment |
3 Answers
3
active
oldest
votes
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
$endgroup$
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
$endgroup$
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
395117
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
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3 Answers
3
active
oldest
votes
3 Answers
3
active
oldest
votes
active
oldest
votes
active
oldest
votes
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
$endgroup$
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
$endgroup$
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
$endgroup$
It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
answered Jan 14 at 19:06
betelgeusebetelgeuse
10612
10612
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
2
2
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
$begingroup$
I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
$endgroup$
– CKM
Jan 14 at 21:10
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
$endgroup$
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
$endgroup$
add a comment |
$begingroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
$endgroup$
I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
answered Jan 15 at 16:56
swbarnes2swbarnes2
3,600811
3,600811
add a comment |
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
395117
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
395117
$endgroup$
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
$begingroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
395117
$endgroup$
For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.
answered Jan 14 at 21:17
CellCell
395117
edited Jan 15 at 1:55
answered Jan 14 at 21:17
CellCell
395117
answered Jan 14 at 21:17
CellCell
395117
answered Jan 14 at 21:17
CellCell
395117
395117
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
$endgroup$
– swbarnes2
Jan 14 at 23:10
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
$begingroup$
Interesting. Thanks for sharing I didn't know that.
$endgroup$
– Cell
Jan 15 at 1:53
add a comment |
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$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51